yokL [2018-11-19 19:20:20]

similar to phage-related protein

locus: BSU21550

pI: 4.78

mw: 20.71 kDa

protein length: 177 aa

gene length: 531 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.2|SP-beta prophage]

Gene

Coordinates: 2,273,989 → 2,274,522

The protein

Protein family

• sleB family (according to Swiss-Prot)

Structure

• [PDB|6DAU] (SpeG from Vibrio cholerae, 27% identity)

Expression and Regulation

Operons

• genes: [gene|13C081250872E72C61C49D8F77AC5AF4B6D431A3|yokI]-[gene|9BC2309D7FC93CD4195849BFCF195ED3EBC3CAF1|yokJ]-[gene|8BA8DB1483190AB847F28F3E56C247FE85C3225E|yokK]-[gene|9A03964C94A9253B3D9AAA60ACA0651AED49A627|yokL]
  description: [Pubmed|20817675]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|yolA]' and '[protein|search|yokL]' [PubMed|20525796]

Biological materials

Mutant

• BKE21550 (Δ[gene|9A03964C94A9253B3D9AAA60ACA0651AED49A627|yokL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE21550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTAACTTTCCCTCCAG, downstream forward: _UP4_TAATATCACTTGTGCATCGA
• BKK21550 (Δ[gene|9A03964C94A9253B3D9AAA60ACA0651AED49A627|yokL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK21550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTAACTTTCCCTCCAG, downstream forward: _UP4_TAATATCACTTGTGCATCGA

References

• 20525796,20817675